confocal microscope zeiss 700 Search Results


spinning disk confocal microscope  (Yokogawa Electric)
99
Yokogawa Electric spinning disk confocal microscope
Spinning Disk Confocal Microscope, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/CSU-10/pm16782006-223-22-33
Average 99 stars, based on 1 article reviews
spinning disk confocal microscope - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
lsm 700 confocal microscope  (3DHistech ltd)
90
3DHistech ltd lsm 700 confocal microscope
Lsm 700 Confocal Microscope, supplied by 3DHistech ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/lsm+700+confocal+microscope/pm25705371-60-5-15
Average 90 stars, based on 1 article reviews
lsm 700 confocal microscope - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
axio observer microscope  (Carl Zeiss)
99
Carl Zeiss axio observer microscope
Axio Observer Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/Microscope+stand+Axio+Observer+3/pmc04912916-178-14-13
Average 99 stars, based on 1 article reviews
axio observer microscope - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
confocal microscope  (Danaher Inc)
99
Danaher Inc confocal microscope
Confocal Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/STELLARIS+5+Confocal+Microscope+Platforms/pmc06364572-161-6-8
Average 99 stars, based on 1 article reviews
confocal microscope - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
fourier transform infrared spectroscopy  (JASCO Inc)
96
JASCO Inc fourier transform infrared spectroscopy
Fourier Transform Infrared Spectroscopy, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/JASCO+Confocal+Raman+Microscope+System/10__1021_slash_jp808676d____jp808676d_si_001-5-28-34
Average 96 stars, based on 1 article reviews
fourier transform infrared spectroscopy - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
dmi8 microscope  (Leica Microsystems)
99
Leica Microsystems dmi8 microscope
Dmi8 Microscope, supplied by Leica Microsystems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/DMi8+S+Inverted+Microscope+Solution/bio_rxiv__2023__01__14__524041-192-12-11
Average 99 stars, based on 1 article reviews
dmi8 microscope - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
denmark mouse monoclonal neurod1 antibody  (Danaher Inc)
99
Danaher Inc denmark mouse monoclonal neurod1 antibody
Average hormone and <t> NeuroD1 </t> expression values
Denmark Mouse Monoclonal Neurod1 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/mouse+monoclonal+Anti-SOX2+antibody/pmc06349459-293-67-76
Average 99 stars, based on 1 article reviews
denmark mouse monoclonal neurod1 antibody - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
oe19 human esophageal adenocarcinoma cell line  (DSMZ)
93
DSMZ oe19 human esophageal adenocarcinoma cell line
Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and <t>OE19</t> (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib
Oe19 Human Esophageal Adenocarcinoma Cell Line, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/OE-19/pm36163066-91-33-47
Average 93 stars, based on 1 article reviews
oe19 human esophageal adenocarcinoma cell line - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
collagen iv  (Thermo Fisher)
99
Thermo Fisher collagen iv
Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and <t>OE19</t> (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib
Collagen Iv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/Collagen/pm31028373-666-184-202
Average 99 stars, based on 1 article reviews
collagen iv - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
eclipse ti2 confocal microscopes  (Nikon)
99
Nikon eclipse ti2 confocal microscopes
Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and <t>OE19</t> (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib
Eclipse Ti2 Confocal Microscopes, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/ECLIPSE+Ti2/pmc12821313-91-9-8
Average 99 stars, based on 1 article reviews
eclipse ti2 confocal microscopes - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
a1 confocal microscope  (Nikon)
99
Nikon a1 confocal microscope
Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and <t>OE19</t> (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib
A1 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/Objectives/pmc09348902-444-61-60
Average 99 stars, based on 1 article reviews
a1 confocal microscope - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
dragonfly spinning disk confocal microscope  (Oxford Instruments)
99
Oxford Instruments dragonfly spinning disk confocal microscope
Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and <t>OE19</t> (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib
Dragonfly Spinning Disk Confocal Microscope, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+microscope+zeiss+700/Dragonfly/pm37159662-267-13-12
Average 99 stars, based on 1 article reviews
dragonfly spinning disk confocal microscope - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


Average hormone and NeuroD1 expression values

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: Average hormone and NeuroD1 expression values

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Expressing

(A) Double stain immunohistochemistry, plurihormonal adenoma, Prolactin/NeuroD1, × 200. Prolactin is visualized with red colour, NeuroD1 with blue colour. Same cell co-expression of Prolactin and NeuroD1 is 11%. Generally, the average number of cells with co-expression (Prolactin/NeuroD1) in this pituitary adenoma is 11.3 ± 7.2%. (B) Double stain immunohistochemistry, plurihormonal adenoma, Growth hormone/NeuroD1, × 200. Growth hormone is visualized with red colour, NeuroD1 with blue colour. Same cell co-expression of Growth hormone/NeuroD1 is 12%. Generally, the average number of cells with co-expression (Growth hormone/NeuroD1) in this pituitary adenoma is 10.0 ± 3.1%.

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: (A) Double stain immunohistochemistry, plurihormonal adenoma, Prolactin/NeuroD1, × 200. Prolactin is visualized with red colour, NeuroD1 with blue colour. Same cell co-expression of Prolactin and NeuroD1 is 11%. Generally, the average number of cells with co-expression (Prolactin/NeuroD1) in this pituitary adenoma is 11.3 ± 7.2%. (B) Double stain immunohistochemistry, plurihormonal adenoma, Growth hormone/NeuroD1, × 200. Growth hormone is visualized with red colour, NeuroD1 with blue colour. Same cell co-expression of Growth hormone/NeuroD1 is 12%. Generally, the average number of cells with co-expression (Growth hormone/NeuroD1) in this pituitary adenoma is 10.0 ± 3.1%.

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Staining, Immunohistochemistry, Expressing

Growth hormone is visualized with red colour, NeuroD1 with blue colour. Same cell co-expression of Growth hormone and NeuroD1 is 100%. Generaliiy, the average number of cells with co-expression (Growth hormone/NeuroD1) in this pituitary adenoma is 99.8%.

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: Growth hormone is visualized with red colour, NeuroD1 with blue colour. Same cell co-expression of Growth hormone and NeuroD1 is 100%. Generaliiy, the average number of cells with co-expression (Growth hormone/NeuroD1) in this pituitary adenoma is 99.8%.

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Expressing

(A) : blue fluorescence of cell nuclei (DAPI); (B) : green fluorescence of Prolactin; (C) : red fluorescence of NeuroD1; (D) : overlay image (A, B, C) . NeuroD1(pink fluorescence)/Prolactin (green fluorescence) same cell co-expression is seen in 30% of the cells (indicated by arrows); × 600; (E) : scatterplot of blue (DAPI, Ch1) and red (Neuro D1, Ch 3) pixel intensities of tumor cell nuclei; (F) : intensity histogram of red (Neuro D1), green (Prolactin), and blue (DAPI) fluorescence. White channel: light microscopy; (G) : blue fluorescence of cell nuclei (DAPI); (H) : green fluorescence of Growth hormone; (I) : red fluorescence of NeuroD1; (J) : overlay image (G, H, I) . NeuroD1(pink fluorescence)/Growth hormone (green fluorescence) same cell co-expression is seen in 77% of the cells (indicated by arrows); × 2400; (K) : scatterplot of blue (DAPI, Ch1) and red (Neuro D1, Ch 3) pixel intensities of tumor cell nuclei; (L) : intensity histogram of red (Neuro D1), green (Growth hormone), and blue (DAPI) fluorescence. White channel: light microscopy.

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: (A) : blue fluorescence of cell nuclei (DAPI); (B) : green fluorescence of Prolactin; (C) : red fluorescence of NeuroD1; (D) : overlay image (A, B, C) . NeuroD1(pink fluorescence)/Prolactin (green fluorescence) same cell co-expression is seen in 30% of the cells (indicated by arrows); × 600; (E) : scatterplot of blue (DAPI, Ch1) and red (Neuro D1, Ch 3) pixel intensities of tumor cell nuclei; (F) : intensity histogram of red (Neuro D1), green (Prolactin), and blue (DAPI) fluorescence. White channel: light microscopy; (G) : blue fluorescence of cell nuclei (DAPI); (H) : green fluorescence of Growth hormone; (I) : red fluorescence of NeuroD1; (J) : overlay image (G, H, I) . NeuroD1(pink fluorescence)/Growth hormone (green fluorescence) same cell co-expression is seen in 77% of the cells (indicated by arrows); × 2400; (K) : scatterplot of blue (DAPI, Ch1) and red (Neuro D1, Ch 3) pixel intensities of tumor cell nuclei; (L) : intensity histogram of red (Neuro D1), green (Growth hormone), and blue (DAPI) fluorescence. White channel: light microscopy.

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Fluorescence, Expressing, Light Microscopy

Prolactin is visualized with red colour, NeuroD1 with blue colour. PRL/NeuroD1 co-expression is seen in 90% of cells.

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: Prolactin is visualized with red colour, NeuroD1 with blue colour. PRL/NeuroD1 co-expression is seen in 90% of cells.

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Expressing

Percentage of antigen expressing cells in normal adenohypophysis fragments taken near adenoma boundaries

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: Percentage of antigen expressing cells in normal adenohypophysis fragments taken near adenoma boundaries

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Expressing

Percentage of antigen expressing cells in normal adenohypophysis

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: Percentage of antigen expressing cells in normal adenohypophysis

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Expressing

Growth hormone is visualized with red colour, NeuroD1 with blue colour. Co-expression (Growth hormone and NeuroD1) is seen in 80% of cells (in this photo; indicated by arrows). GH/NeuroD1 same cell co-expression was 45%, on average in this case.

Journal: Oncotarget

Article Title: Analysis of pituitary adenoma expression patterns suggests a potential role for the NeuroD1 transcription factor in neuroendocrine tumor-targeting therapies

doi: 10.18632/oncotarget.26513

Figure Lengend Snippet: Growth hormone is visualized with red colour, NeuroD1 with blue colour. Co-expression (Growth hormone and NeuroD1) is seen in 80% of cells (in this photo; indicated by arrows). GH/NeuroD1 same cell co-expression was 45%, on average in this case.

Article Snippet: For immunohistochemical staining, confocal microscopy, and electron immunocytochemistry, the following primary antibodies were used: mouse monoclonal ACTH antibody, diluted 1:500 (clone AH26, Diagnostic BioSystems, Netherlands) rabbit polyclonal TSH antibody, RTU (Cell Marque, USA) mouse monoclonal FSH antibody, diluted 1:100 (clone С10, DAKO, Denmark) mouse monoclonal LH antibody, diluted 1:500 (clone С93, DAKO, Denmark) rabbitpolyclonal GH antibody, diluted 1:100 (BioGenex, USA) rabbit polyclonal PRL antibody, diluted 1:700 (DAKO, Denmark) mouse monoclonal NeuroD1 antibody, diluted 1:1000 (clone ab60704, Abcam, United Kingdom) mouse monoclonal Ki-67antibody, diluted 1:200 (clone MIB-1, DAKOCytomation, Denmark) mouse monoclonal CK7antibody, diluted 1:300 (clone OV-TL 12/30, DAKO, Denmark) The following secondary antibodies/reagents were used for immunohistochemical staining: mouse EnVisionTM+ System, Peroxidase (DAKO, Denmark) rabbit EnVisionTM+ System, Peroxidase (DAKO, Denmark) MultiVision Polymer Cocktail (Thermo Scientific, UK) The following secondary antibodies were used for confocal microscopy: Alexa Fluor 647 goat anti-Mouse, diluted 1:100 (Abcam, UK) Alexa Fluor 488 goat anti-Rabbit, diluted 1:100 (Abcam, UK) The following secondary antibodies were used for electron immunocytochemistry: goat-anti mouse antibody conjugated to 10nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US) goat-anti rabbit antibody conjugated to 5nm colloidal gold, diluted 1:100 (Sigma-Aldrich, US)

Techniques: Expressing

Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and OE19 (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Gasdermin B over-expression modulates HER2-targeted therapy resistance by inducing protective autophagy through Rab7 activation.

doi: 10.1186/s13046-022-02497-w

Figure Lengend Snippet: Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and OE19 (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib

Article Snippet: Cell culture and in vitro assays HCC1954 (derived from a human invasive ductal breast carcinoma), NCI-N87 (human gastric adenocarcinoma) and HEK293T cell lines were obtained from the American Type Cell Culture (ATCC) and OE19 (human esophageal adenocarcinoma) cell line from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ).

Techniques: Expressing, Transmission Assay, Electron Microscopy, Western Blot, Confocal Microscopy, Two Tailed Test, Control